Recombinant Human Neuroligin 4/NLGN4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Neuroligin 4/NLGN4 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Neuroligin 4X/NLGN4X protein
  Gln42-Ser676, with a C-terminal 6-His tag
• Accession #: NP_065793
• N-terminal Sequence: No results obtained: Gln42 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: NLGN4X
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neuroligin 4/NLGN4 Protein, CF

• ASPGX2
• AUTSX2
• HLNX
• HNL4X
• HNLX
• KIAA1260AUTSX2
• MGC22376
• neuroligin 4, X-linked
• Neuroligin 4X
• NL4
• NLGN
• NLGN4
• NLGN4neuroligin 4
• NLGN4X
• X-linked

Background

Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type-B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1 - 3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X-chromosome. It shares 69% - 73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta -neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium-dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8 - 10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).

1. Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
2. Chih, B. et al. (2005) Science 307:1324.
3. Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
4. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
5. Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
6. Fabrichny, I. P. et al. (2007) Neuron 56:979.
7. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
8. Jamain, S. et al. (2003) Nat. Genet. 34:27.
9. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
10. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
11. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.

Publications for Neuroligin 4X/NLGN4X (5158-NL)(2)

Publications using 5158-NL

• T Yumoto, M Kimura, R Nagatomo, T Sato, S Utsunomiya, N Aoki, M Kitaura, K Takahashi, H Takemoto, H Watanabe, H Okano, F Yoshida, Y Nao, T Tomita Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms Mol Autism, 2020-09-01;11(1):68. 2020-09-01 [PMID: 32873342] (Bioassay, In Vivo, Rat)
• HS Venkatesh, LT Tam, PJ Woo, J Lennon, S Nagaraja, SM Gillespie, J Ni, DY Duveau, PJ Morris, JJ Zhao, CJ Thomas, M Monje Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma Nature, 2017-09-20;549(7673):533-537. 2017-09-20 [PMID: 28959975] (Bioassay, Human)

Bioinformatics

• Gene Symbol: NLGN4X
• Uniprot:
  • NP_065793()