Recombinant Human MDL-1/CLEC5A Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MDL-1/CLEC5A Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑9 (Catalog # 2045-GA) is coated at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human MDL-1/CLEC5A that produces 50% optimal binding response is typically 3-18 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human MDL-1/CLEC5A protein
  Tyr26-Lys188, with an N-terminal 9-His tag
• Accession #: Q9NY25
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLEC5A
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MDL-1/CLEC5A Protein, CF

• CLEC5A
• CLECSF5
• CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5
• C-type lectin domain family 5, member A
• C-type lectin superfamily member 5
• MDL1
• MDL-1
• MDL-1MGC138304
• Myeloid DAP12-associating lectin 1
• myeloid DAP12-associating lectin-1

Background

MDL-1 (Myeloid DAP12-associating lectin 1), also known as CLEC5A, is an approximately 40 kDa member of the C-type lectin family (1). Mature human MDL-1 is a glycosylated type 2 transmembrane protein that associates into a homodimer on the cell surface (2). It consists of a 161 amino acid (aa) extracellular domain (ECD) with one C-type lectin (CTL) domain and a juxtamembrane stalk region, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (3). Within the ECD, human MDL-1 shares 67% and 69% aa sequence identity with mouse and rat MDL-1, respectively. The transmembrane segment contains a lysine residue that mediates interactions with the signaling protein DAP12 (3). MDL-1 is expressed on monocytes, macrophages, and neutrophils (3, 4). Its expression is up-regulated on monocytes in rheumatoid arthritis (5). MDL-1 functions as a cell attachment receptor for all four serotypes of Denguevirus as well as Japanese encephalitis virus, although the short isoform binds significantly more weakly (2, 6, 7). These interactions trigger DAP12 phosphorylation, the production of multiple inflammatory cytokines, vascular leakage, and disruption of the blood-brain barrier (6-8).

1. Hoving, J.C. et al. (2014) Cell. Microbiol. 16:185.
2. Watson, A.A. et al. (2011) J. Biol. Chem. 286:24208.
3. Bakker, A.B.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9792.
4. Aoki, N. et al. (2009) J. Leukoc. Biol. 85:508.
5. Chen, D.-Y. et al. (2014) PLoS ONE 9:e86105.
6. Chen, S.-T. et al. (2012) PLoS Pathogens 8:e1002655.
7. Chen, S.-T. et al. (2008) Nature 453:672.
8. Wu, M.-F. et al. (2013) Blood 121:95.

Publications for MDL-1/CLEC5A (8544-CL)(1)

Publication using 8544-CL

• HW Wang, HH Li, SC Wu, CK Tang, HY Yu, YC Chang, PS Sung, WL Liu, MP Su, GY Yu, LR Huang, CH Chen, SL Hsieh CLEC5A mediates Zika virus-induced testicular damage Journal of Biomedical Science, 2023-02-17;30(1):12. 2023-02-17 [PMID: 36803804] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CLEC5A
• Uniprot:
  • Q9NY25()