Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

When Recombinant Human LILRA2/CD85h/ILT1 (Catalog # 9040-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) binds with a typical ED50 of150-900 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LILRA2/CD85h/ILT1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA2/CD85h/ILT1 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7. (Catalog # 914-AN) binds with a typical ED₅₀ of 150-900 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human LILRA2/CD85h/ILT1 protein
  Gly24-Asn449, with a C-terminal 6-His tag
• Accession #: NP_001124389
• N-terminal Sequence: Gly24
• Protein/Peptide Type: Recombinant Proteins
• Gene: LILRA2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-81 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

• CD85 antigen-like family member H
• CD85h antigen
• CD85h
• ILT1
• ILT-1
• ILT1CD85H
• Leukocyte immunoglobulin-like receptor 7
• leukocyte immunoglobulin-like receptor subfamily A member 2 soluble
• leukocyte immunoglobulin-like receptor subfamily A member 2
• leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2
• LILRA2
• LIR7
• LIR7LIR-7Immunoglobulin-like transcript 1

Background

LILRA2, also known as ILT1, CD85h, and LIR7, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA2 consists of a 426 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail (2). Alternative splicing generates isoforms with short deletions between the fourth Ig-like domain and the transmembrane region, and an isoform that is truncated C-terminal to the fourth Ig-like domain (3, 4). LILRA2 is expressed on monocytes, neutrophils, basophils, and eosinophils (5-7). It contains a positively charged  arginine residue in its transmembrane segment, enabling association with the signaling protein Fc epsilon RI gamma (5). Cross-linking of LILRA2 on monocytes induces the production of multiple cytokines as well as the upregulation of Fc gamma receptors (6, 7). Cross-linking also restricts monocyte differentiation into immature dendritic cells, phagocytic activity, and antigen presentation to T cells (6, 7). R&D Systems in-house testing indicates that LILRA2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families.

1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
2. Borges, L. et al. (1997) J. Immunol. 159:5192.
3. Jones, D.C. et al. (2009) Eur. J. Immunol. 39:3195.
4. Mamegano, K. et al. (2008) Genes Immun. 9:214.
5. Nakajima, H. et al. (1999) J. Immunol. 162:5.
6. Lu, H.K. et al. (2012) PLoS One 7:e33478.
7. Lee, D.J. et al. (2007) J. Immunol. 179:8128.

Bioinformatics

• Gene Symbol: LILRA2
• Uniprot:
  • NP_001124389()