Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human KIR2DS4/CD158i protein
  

  Human KIR2DS4 (Gln22-His245) Accession # P43632	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P43632
• N-terminal Sequence: No results obtained: Gln22 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: KIR2DS4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF

• CD158 antigen-like family member I
• CD158i antigen
• CD158i
• cl-39
• killer cell immunoglobulin-like receptor 2DS4
• killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8
• killer inhibitory receptor 4-1-2
• KIR antigen 2DS4
• KIR2DS4
• KKA3KIR1D
• MGC120019
• MGC125315
• MGC125317
• MHC class I NK cell receptor
• natural killer cell inhibitory receptor
• Natural killer-associated transcript 8
• NKAT-8
• NKAT8KIR412
• P58 natural killer cell receptor clones CL-39/CL-17
• p58 NK receptor CL-39/CL-17

Background

KIR2DS4 (2DS4), previously called NKAT-8 and designated CD158i, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1). KIRs are expressed on CD56^dim NK cells and T cell subsets where they differentiate normal from abnormal cells and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. KIR2DS4 cDNA encodes 304 amino acids (aa) including a 21 aa signal sequence, a 224 aa ECD, a 20 aa transmembrane sequence and a 39 aa cytoplasmic domain. KIR receptors have no structural orthologs in nonprimates, although mouse Ly-49 proteins perform similar functions (2). Like other activating KIRs, KIR2DS4 has a short tail and a positively charged amino acid (aa) within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12 (2, 3). Expression of activating KIR is balanced with inhibitory KIR to impart specificity to the immune response. KIR2DS4 recognizes six HLA-C allotypes and two HLA-A allotypes (A*1101 and A*1102) but no HLA-B allotypes (4, 5). It has also been suggested that KIR2DS4 recognizes a non-MHC ligand, based on specific binding to melanoma cells that lack MHC Class I (6). A common allele, KIR2DS4*003, is present in up to 89% of Caucasians and ~30% of Asians; it creates a prematurely terminated, soluble, inactive form (7, 8). Lack of functional KIR2DS4 can potentially create unbalanced inhibition of killer cell function (4, 8).

1. Bottino, C. et al. (1996) Eur. J. Immunol. 26:1816.
2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
3. Purdy, A.K. and K.S. Campbell (2009) Cancer Biol. Ther. 8:13.
4. Graef, T. et al. (2009) J. Exp. Med. 206:2557.
5. Katz, G. et al. (2001) J. Immunol. 166:7260.
6. Katz, G. et al. (2004) J. Immunol. 173:1819.
7. Maxwell, L.D. et al. (2004) Hum. Immunol. 65:613.
8. Middleton, D. et al. (2007) Hum. Immunol. 68:128.

Publications for KIR2DS4/CD158i (1847-KR)(2)

Publications using 1847-KR

• MJW Sim, S Rajagopala, DM Altmann, RJ Boyton, PD Sun, EO Long Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C Proc. Natl. Acad. Sci. U.S.A., 2019-05-28;0(0):. 2019-05-28 [PMID: 31138701] (Flow Cytometry, Human)
• S Ryser, A Estellés, E Tenorio, LM Kauvar, ML Gishizky High affinity anti-TIM-3 and anti-KIR monoclonal antibodies cloned from healthy human individuals PLoS ONE, 2017-07-19;12(7):e0181464. 2017-07-19 [PMID: 28723950] (Bioassay, Human)

Bioinformatics

• Gene Symbol: KIR2DS4
• Uniprot:
  • P43632()