Recombinant Human ISM1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human ISM1 Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Isthmin 1/ISM1, immobilized at 5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human Isthmin 1/ISM1 protein
Ser27-Tyr464, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser27
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
62-71 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ISM1 Protein, CF

  • bA149I18.1
  • C20orf82
  • Chromosome 20 Open Reading Frame 82
  • dJ1077I2.1
  • ISM
  • ISM1
  • ISM-1
  • Isthmin 1 Homolog (Zebrafish)
  • Isthmin 1 Homolog
  • Isthmin 1
  • Isthmin
  • Isthmin-1

Background

Isthmin 1 (ISM1) is an adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). The secreted human ISM1 consists of a 45 amino acid (aa) TSR domain, and a 164 aa AMOP domain. Within these domains, human ISM1 shares 92% and 93% aa sequence identity with mouse and rat ISM1, respectively (2). ISM1 shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, ISM1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of ISM1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble ISM1 induced endothelial cell apoptosis (6). In mouse, ISM1 is mainly expressed in the epithelium of bronchi and alveoli of the lung as well as in the cerebral cortex and hippocampus of the brain, sugggesting the relevance of this protein for the development and homeostasis of these organs (4). Our in-house bioassay has showed that ISM1 can support cortical neuron neurite outgrowth.
  1. Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
  2. Valle-Rios, R. et al. (2014) J. Interferon Cytokine Res. 34:795.
  3. Lansdon, L. et al. (2018). Genetics. 208:283.
  4. Osório, L. et al. (2014). Cell Cycle. 13:1571.
  5. Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
  6. Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.

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Bioinformatics

Entrez
Uniprot