Recombinant Human ISM1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ISM1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Isthmin 1/ISM1 protein
  Ser27-Tyr464, with a C-terminal 6-His tag
• Accession #: B1AKI9
• N-terminal Sequence: Ser27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-71 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ISM1 Protein, CF

• bA149I18.1
• C20orf82
• Chromosome 20 Open Reading Frame 82
• dJ1077I2.1
• ISM
• ISM1
• ISM-1
• Isthmin 1 Homolog (Zebrafish)
• Isthmin 1 Homolog
• Isthmin 1
• Isthmin
• Isthmin-1

Background

Isthmin 1 (ISM1) is an adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). The secreted human ISM1 consists of a 45 amino acid (aa) TSR domain, and a 164 aa AMOP domain. Within these domains, human ISM1 shares 92% and 93% aa sequence identity with mouse and rat ISM1, respectively (2). ISM1 shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, ISM1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of ISM1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble ISM1 induced endothelial cell apoptosis (6). In mouse, ISM1 is mainly expressed in the epithelium of bronchi and alveoli of the lung as well as in the cerebral cortex and hippocampus of the brain, sugggesting the relevance of this protein for the development and homeostasis of these organs (4). Our in-house bioassay has showed that ISM1 can support cortical neuron neurite outgrowth.

1. Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
2. Valle-Rios, R. et al. (2014) J. Interferon Cytokine Res. 34:795.
3. Lansdon, L. et al. (2018). Genetics. 208:283.
4. Osório, L. et al. (2014). Cell Cycle. 13:1571.
5. Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
6. Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.

Bioinformatics

• Uniprot:
  • B1AKI9()