Recombinant Human ISLR-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ISLR-2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 200-800 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human ISLR-2 protein
  Cys20-Leu590, with a C-terminal 6-His tag
• Accession #: Q6UXK2
• N-terminal Sequence: Cys20
• Protein/Peptide Type: Recombinant Proteins
• Gene: ISLR2
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ISLR-2 Protein, CF

• immunoglobulin superfamily containing leucine-rich repeat 2
• ISLR2
• ISLR-2
• KIAA1465
• leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein
• LINX
• UNQ1885/PRO4329

Background

ISLR2 (Immunoglobulin Superfamily containing Leucine-rich Repeat protein 2; also known as LINX/Leucine-rich repeat domain and Immunoglobulin domain containing axoN eXtension protein and Mbu-3 [in mouse]) is a member of the LIG superfamily of molecules (1, 2). Members of this family are typically integral membrane proteins that are characterized by the presence of multiple LRRs (leucine-rich repeats) N-terminal to one Ig domain.  ISLR2 should not be confused with an identically‑named, widely-expressed transcript isoform containing the ISLR1 protein (3). ISLR2 is principally associated with the embryonic nervous system.  In particular, it appears to coexist with TRKs on both sensory and motor postmitotic neurons (2, 4, 5). On these spinal neuron cell types, ISLR2 (plus other LIG family members) is suggested to fine-tune TrkA, TrkC and Ret downstream signaling in a temporally-restricted manner. This is posited to impact axonal outgrowth, branching and fasciculation (5). Notably, a lack of ISLR2 does not abrogate TrkA signaling. This suggests a modulating role for the molecule, and is consistent with the observation that ISLR2 and Trk interact through their extracellular domains (4, 5). Mature human ISLR2 is a 100-120 kDa, 727 amino acid (aa) type I transmembrane protein. It contains a 571 aa extracellular region (aa 19-589) plus a 135 aa cytoplasmic domain (2, 6). The extracellular region possesses seven LRR repeats (aa 19‑232) coupled to one Ig-like domain (aa 233-371).  Human and mouse extracellular domains share 89% aa sequence  identity (6).

1. MacLaren, C.M. et al. (2004) Dev. Genes Evol. 214:360.
2. Gejima, R. et al. (2006) Gene Expr. Patterns 6:235.
3. Nagasawa, A. et al. (1999) Genomics 61:37.
4. Homma, S. et al. (2009) Gene Expr. Patterns 9:1.
5. Mandai, K. et al. (2009) Neuron 63:614.
6. Lee, C.J. et al. (2008) BMB Rep. 41:875.

Bioinformatics

• Gene Symbol: ISLR2
• Uniprot:
  • Q6UXK2()