Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF

When Recombinant Human IL-36 beta /IL-1F8 (6834-ILB/CF) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein (Catalog # AVI11116) binds with an ED50 of 1.50-15.0 μg/mL.

2 μg/lane of Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein (Catalog # AVI11116) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-87 kDa and 156-174 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-36 beta /IL-1F8 (Catalog # 6834-ILB/CF) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag (Catalog # AVI11116) binds with an ED₅₀ of 1.50-15.0 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL-1Rrp2/IL-1R6 protein
  

  Human IL-1 R6 (Asp20-Tyr337) Accession # Q9HB29.2	DIEGRMD	Human IgG1 Fc (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: Q9HB29.2
• N-terminal Sequence: Protein identity confirmed by mass spectrometry.
• Structure / Form: Disulfide-linked homodimer
  Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 65 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 78-87 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF

• IL-1 R6
• IL-1 Rrp2
• IL1RL2
• IL-1RL2
• IL1Rrp2
• IL-1Rrp2
• IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2
• interleukin 1 receptor-like 2
• interleukin-1 receptor-like 2

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1Rrp2 (IL-1 RL2/IL-1R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1Rrp2, respectively. IL-1Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a cofactor (3, 4). Response to IL-1F9 is specifically antagonized by IL-1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1Rrp2, IL-1 F5, and IL-1F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1Rrp2 mediated signaling pathway may take part in local inflammatory responses (3). Our Avi-tag Biotinylated IL-1Rrp2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity

 

1. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
2. Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
3. Debets, R. et al. (2001) J. Immunol. 167:1440.
4. Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.

Bioinformatics

• Uniprot:
  • Q9HB29.2()