Recombinant Human IFN-gamma R1 Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IFN-gamma R1 Protein Summary

• Details of Functionality: Measured by its ability to inhibit rhIFN-gamma mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED 50 for this effect is 1-3 µg/mL in the presence of 2 ng/mL rhIFN-gamma .
• Source: Mouse myeloma cell line, NS0-derived human IFN-gamma R1/CD119 protein Met1-Gly245
• Accession #: P15260.1
• N-terminal Sequence: Glu18 & Gly20
• Protein/Peptide Type: Recombinant Proteins
• Gene: IFNGR1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40 kDa and 50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma R1 Protein

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Publications for IFN-gamma R1/CD119 (673-IR)(4)

Publications using 673-IR

• J Kleemann, K Steinhorst, V König, N Zöller, J Cinatl, D Özistanbul, R Kaufmann, M Meissner, S Kippenberg Functional Downregulation of PD-L1 and PD-L2 by CpG and non-CpG Oligonucleotides in Melanoma Cells Cancers, 2022-09-27;14(19):. 2022-09-27 [PMID: 36230620] (Cell Culture, Human)
• Carlos M Minutti Surfactant Protein A Prevents IFN-?/IFN-? Receptor Interaction and Attenuates Classical Activation of Human Alveolar Macrophages J Immunol, 2016-06-06;0(0):. 2016-06-06 [PMID: 27271568] (Bioassay, Human)
• Wong J, Muthuswamy R, Bartlett D, Kalinski P IL-18-based combinatorial adjuvants promote the intranodal production of CCL19 by NK cells and dendritic cells of cancer patients. Oncoimmunology, 2013-09-12;2(9):e26245. 2013-09-12 [PMID: 24228233] (Bioassay, Human)
• Sarrazin S, Bonnaffe D, Lubineau A, Lortat-Jacob H Heparan sulfate mimicry: a synthetic glycoconjugate that recognizes the heparin binding domain of interferon-gamma inhibits the cytokine activity. J. Biol. Chem., 2005-09-09;280(45):37558-64. 2005-09-09 [PMID: 16155294] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: IFNGR1
• Uniprot:
  • P15260.1()