Recombinant Human His6-USP19 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP19 Protein, CF Summary

• Details of Functionality:

  Recombinant Human His6-USP19 is a ubiquitin­-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP19 concentration of 10-100 nM.

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP19 protein
  Met1 - Arg1290, Contains a C-terminal 6-His tag
• Accession #: O94966
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP19
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Theoretical MW: 143 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP19 Protein, CF

• Deubiquitinating enzyme 19
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA0891
• Ubiquitin Carboxyl-Terminal Hydrolase 19
• ubiquitin specific peptidase 19
• ubiquitin thioesterase 19
• Ubiquitin Thiolesterase 19
• Ubiquitin-specific-processing protease 19
• USP19
• Zinc finger MYND domain-containing protein 9
• ZMYND9
• ZMYND9ubiquitin specific protease 19

Background

Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family that contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR) delta F508 and T-cell receptor-alpha (TCR alpha ) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCR alpha (but not CFTR delta F508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids a C-terminal 6-His tag.

1. Hassink G.C., et al. (2009) EMBO Rep. 10: 755
2. Iphöfer, A. et al. (2012) ChemBioChem 13: 1416
3. Mei, Y. et al. (2011) J. Biol. Chem. 286: 35380

Bioinformatics

• Gene Symbol: USP19
• Entrez:
  • 10869(Human)
  • 71472(Mouse)
  • 361190(Rat)
• Uniprot:
  • O94966()