Details of Functionality | Recombinant Human His6-USP19 is a ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP19 concentration of 10-100 nM. |
Source | Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP19 protein Met1 - Arg1290, Contains a C-terminal 6-His tag |
Accession # | |
Protein/Peptide Type | Recombinant Enzymes |
Gene | USP19 |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Dilutions |
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Theoretical MW | 143 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP |
Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family that contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR) delta F508 and T-cell receptor-alpha (TCR alpha ) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCR alpha (but not CFTR delta F508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids a C-terminal 6-His tag.
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