Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Hepassocin/FGL1 protein
MD
Human IgG1
(Pro100-Lys330)
IEGR Human Hepassocin/FGF1
(Leu23-Ile312)
Accession # Q08830.3
N-terminusC-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-65 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

  • FGL1
  • fibrinogen-like 1
  • fibrinogen-like protein 1
  • Fibrinogen-like-protein 1
  • FREP1
  • Hepassocin
  • hepatocellular carcinoma-related sequence
  • Hepatocyte-derived fibrinogen-related protein 1
  • HFREP-1
  • HFREP1MGC12455
  • HP-041
  • LFIRE1
  • LFIRE-1
  • Liver fibrinogen-related protein 1

Background

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).
  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

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