Recombinant Human GPR56 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human GPR56 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin. When 4 x 104 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL, Catalog # 1918-FN) and rhGPR56 (10 µg/well, 100 µL/well) for 45 minutes at 37° C there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR56.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR56 protein
Arg26-Val342, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg26
Protein/Peptide Type
Recombinant Proteins
Gene
ADGRG1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
65-85 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GPR56 Protein, CF

  • BFPP
  • EGF-TM7-like
  • G protein-coupled receptor 56,7-transmembrane protein with no EGF-like N-terminal domains-1
  • GPR56
  • Protein TM7XN1
  • TM7LN4
  • TM7LN4G-protein coupled receptor 56
  • TM7XN1
  • TM7XN1DKFZp781L1398

Background

GPR56 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) with long extracellularN-termini (1 - 3). The 693 amino acid (aa) human GPR56 contains a 25 aa signal sequence, a 377 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk followed by a GPCR proteolytic cleavage site (GPS) (1, 4). Cleavage of the 60 kDa N-terminus from the 80 kDa full length form is needed for efficient cell surface expression (5, 6). While the cleaved portion may remain non-covalently associated, it has also been found in conditioned medium of cultured cells (5). Human GPR56 shares 71%, 72%, 80%, 80% and 79% aa identity with mouse, rat, canine, equine, and bovine GPR56 within the cleaved ECD. A functional splice variant lacking the GPS site and a non-functional splice variant lacking portions of the TM domains have also been described (4). A human brain developmental disorder, bilateral frontoparietal polymicrogyria, is associated with GPR56 mutations that also show impaired GPS cleavage, intracellular trafficking, and expression at the cell surface (5). GPR56 is widely distributed, with highest mRNA or expressed sequence tag expression in brain, thyroid, skin and female reproductive system (3, 4). GPR56 expression is upregulated during cell transformation and is high in melanomas, glioblastomas and astrocytomas, but downregulated in melanomas with high metastatic potential (2, 6 - 8). Although the function of GPR56 is not completely known, it is clearly an adhesion protein (6 - 8). Tissue transglutaminase (TG2) is one reported ligand, binding of which inhibits melanoma growth and metastasis (6). Association of GPR56 with the tetraspanin CD81 stabilizes its complex with Gaq/11 for cell signaling (9).

  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Zendman, A.J.W. et al. (1999) FEBS Lett. 446:292.
  3. Liu, M. et al. (1999) Genomics 55:296.
  4. Bjarnadottir, T.K. et al. (2007) Gene 387:38.
  5. Jin, Z. et al. (2007) Hum. Mol. Genet. 16:1972.
  6. Xu, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:9023.
  7. Shashidhar, S. et al. (2005) Oncogene 24:1673.
  8. Ke, N. et al. (2007) Mol. Cancer Ther. 6:1840.
  9. Little, K.D. et al. (2004) Mol. Biol. Cell 15:2375.

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Bioinformatics

Gene Symbol ADGRG1
Uniprot