Recombinant Human G6b Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human G6b Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When 1 μg/mL of rhG6b/Fc Chimera is captured by a goat anti-human IgG Fc Chimera coated plate, the concentration of biotinylated heparin that produces 50% of the optimal binding response is found to be approximately 10-40 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human G6b protein
  

  Human G6b (Asn18-Gln142) Accession # O95866	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O95866
• N-terminal Sequence: Asn18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: C6orf25
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 40.0 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-55 kDa under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human G6b Fc Chimera Protein, CF

• C6orf25
• chromosome 6 open reading frame 25
• G6b
• immunoglobulin receptor
• MGC142279
• MGC142281
• NG31
• protein G6b

Background

G6b is a type I transmembrane glycoprotein in the immunoglobulin superfamily that is encoded within the human class III major histocompatibility complex (1-3). It is expressed mainly on platelets, and has also been detected on megakaryocytes and the human cell line K562 (2-5). Crosslinking of G6b inhibits agonist-stimulated platelet aggregation and activation (5). The 241 amino acid (aa) human G6b isotype B (G6b-B) contains a 17 aa signal sequence, a 125 aa extracellular domain with one V-type Ig-like domain and one N-linked glycosylation site, a 21 aa transmembrane sequence and a 78 aa cytoplasmic sequence with two ITIM inhibitory motifs and a SH3 binding site (1, 2). Phosphorylation at Y211 within the first ITIM is required for interaction with SHP adaptor/tyrosine phosphatase proteins (2, 3). Of several described isoforms, all are identical within aa 22-136, which includes most of the extracellular domain. One contains an alternate signal sequence, while at least two lack a transmembrane sequence and are secreted (2). At least two others, including G6b-A, express alternate C-termini that lack the ITIM motifs. Of the known isoforms, G6b-A and G6b-B predominate in platelets. It is possible that G6b-A may be paired with and control inhibition by G6b-B (4). Heparin interacts with the G6b extracellular domain with high-affinity and may contribute to G6b activity or adhesion to extracellular matrix (6).

1. Ribas, G. et al. (1999) J. Immunol. 163:278.
2. DeVet, E. C. J. M. et al. (2001) J. Biol. Chem. 276:42070.
3. Senis, Y. A. et al. (2007) Mol. Cell. Proteomics 6:548.
4. Macaulay, I. C. et al. (2007) Blood 109:3260.
5. Newland, S. A. et al. (2007) Blood 109:4806.
6. DeVet, E. C. J. M. et al. (2005) FEBS Lett. 579:2355.

Bioinformatics

• Gene Symbol: C6orf25
• Uniprot:
  • O95866(Human)