Recombinant Human Fucosyltransferase 7/FUT7 Protein, CF

Images

 
There are currently no images for Fucosyltransferase 7/FUT7 (6409-GT).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Fucosyltransferase 7/FUT7 Protein, CF Summary

Additional Information
FUT7 has been formulated so that it can be used in the cell surface glycoengineering of living cells, and does not affect cell viability or native phenotype apart from the intended impact on cell glycobiology.
Details of Functionality
Measured by its ability to transfer fucose from GDP-fucose to fetal bovine fetuin. The specific activity is >175 pmol/min/μg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human Fucosyltransferase 7/FUT7 protein
Ala36-Ala342 with a C-terminal 6‑His tag
Accession #
N-terminal Sequence
Ala36
Protein/Peptide Type
Recombinant Enzymes
Gene
FUT7
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-45 kDa, reducing conditions
Publications
Read Publications using
6409-GT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Assay Procedure
  • Assay Buffer: 100 mM Tris, 10 mM MnCl2, 5 mM CaCl2, pH 7.5
  • Recombinant Human Fucosyltransferase 7/FUT7 (rhFUT7) (Catalog # 6409-GT)
  • Donor Substrate: GDP-Fucose (Sigma, Catalog # G4401), 1.6 mM stock in deionized water
  • Acceptor Substrate: Fetuin, from Fetal Bovine Serum (Sigma, Catalog # F3385), 50 mg/mL in deionized water
  • Glycosyltransferase Activity Kit (Catalog # EA001)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute Coupling Phosphatase 1 to 0.1 mg/mL in Assay Buffer.
  2. Prepare reaction mixture by combining 30 µL of 1.6 mM GDP-Fucose, 120 µL of 50 mg/mL Fetuin, 24 µL of 0.1 mg/mL Coupling Phosphatase 1 and 246 µL of Assay Buffer. This volume is sufficient to assay 10 wells.
  3. Dilute rhFUT7 to 5 µg/mL in Assay Buffer.
  4. Dilute 1 mM Phosphate Standard by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 μM stock.
  5. Prepare standard curve by performing seven one-half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.039 to 2.5 nmol per well.
  6. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
  7. Load 15 µL of the 5 µg/mL rhFUT7 into the plate. Include a Control containing 15 µL of Assay Buffer.
  8. Add 35 µL of reaction mixture (step 2) to the wells, excluding the standard curve and curve blank.
  9. Cover the plate with a plate sealer and incubate at 37 °C for 30 minutes.
  10. Add 30 µL of the Malachite Green Reagent A to all wells. Mix briefly.
  11. Add 100 µL of deionized water to all wells. Mix briefly.
  12. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  13. Read plate at 620 nm (absorbance) in endpoint mode.
    Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

Per Reaction:
  • rhFUT7: 0.075 µg
  • Coupling Phosphatase 1: 0.2 µg
  • Fetuin: 500 µg
  • GDP-Fucose: 4000 pmol

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fucosyltransferase 7/FUT7 Protein, CF

  • alpha-(1,3)-fucosyltransferase
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • FTVII
  • fucosyltransferase 7 (alpha (1,3) fucosyltransferase)
  • Fucosyltransferase 7
  • Fucosyltransferase VII
  • Fuc-TVII
  • FucT-VII
  • FUT7
  • Galactoside 3-L-fucosyltransferase
  • Selectin ligand synthase
  • selectin-ligand synthase

Background

Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation.

FUT7 plays an exclusive role for the biosynthesis of sialy Lewis X (sLeX) epitope (NeuAc alpha 2,3Gal beta 1,4 [Fuc alpha 1,3] GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets, and it is critical for the extravasation of immune cells (4, 5, 6). The activity of this enzyme has been measured with a phosphatase-coupled method (7).

R&D Systems Recombinant Human FUT7 has been used for the cell surface glycoengineering of several cell types. In both B cells and mesenchymal stem cells, FUT7 generated, cell surface sLeX leads to enhanced engagement with E-Selectin ligands (8, 9). In naïve regulatory T cells (Treg), engineered sLeX promotes homing to areas of inflammation in vivo (10). These studies suggest that FUT7-mediated generation of sLeX has potential to increase the efficacy of cellular-based therapeutics by enhanced targeting of cells to areas of pathology.

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  4. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  5. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  6. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.
  8. Silva, M. et al. (2017) J. Immunol. 198:3576.
  9. Pachón-Peña, G. et al. (2017) Stem Cells 35:1080.
  10. Donnelly, C. et al. (2018) Sci. Rep. 420:doi:10.1038/s41598-017-17981-z.

Customers Who Viewed This Item Also Viewed...

NB100-1831
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
BBA16
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-78039
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-CS
AF728
Species: Hu
Applications: WB, AdBlk
NB500-526
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-55006
Species: Hu
Applications: ICC/IF
NBP2-14921
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31898
Species: Hu
Applications: ICC/IF, IHC, IHC-P
4949-GT
Species: Hu
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-43299
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
6507-IL/CF
Species: Hu
NBP2-57960
Species: Hu
Applications: ICC/IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF7248
Species: Hu
Applications: WB, IHC, ICC
NBP1-69565
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
6409-GT
Species: Hu
Applications: Enzyme Activity

Publications for Fucosyltransferase 7/FUT7 (6409-GT)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Click Chemistry.


Filter By Application
Bioassay
(4)
Click Chemistry
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species

Reviews for Fucosyltransferase 7/FUT7 (6409-GT) (0)

There are no reviews for Fucosyltransferase 7/FUT7 (6409-GT). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Fucosyltransferase 7/FUT7 (6409-GT) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Fucosyltransferase 7/FUT7 (6409-GT)

Discover related pathways, diseases and genes to Fucosyltransferase 7/FUT7 (6409-GT). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fucosyltransferase 7/FUT7 (6409-GT)

Discover more about diseases related to Fucosyltransferase 7/FUT7 (6409-GT).
 

Pathways for Fucosyltransferase 7/FUT7 (6409-GT)

View related products by pathway.

PTMs for Fucosyltransferase 7/FUT7 (6409-GT)

Learn more about PTMs related to Fucosyltransferase 7/FUT7 (6409-GT).

Blogs on Fucosyltransferase 7/FUT7

There are no specific blogs for Fucosyltransferase 7/FUT7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4
6507-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Fucosyltransferase 7/FUT7 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FUT7
Uniprot