Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF

Images

 
There are currently no images for ErbB3/Her3 (348-RB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5-6.0 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).
Source
Mouse myeloma cell line, NS0-derived human ErbB3/Her3 protein
Human ErbB3
(Ser20-Thr643)
Accession # P21860
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ERBB3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
96 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
130-140 kDa, reducing conditions
Publications
Read Publications using
348-RB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Customers Who Viewed This Item Also Viewed...

AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
1129-ER
Species: Hu
Applications: BA
NBP1-33120
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
236-EG
Species: Hu
Applications: BA
396-HB/CF
Species: Hu
Applications: BA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
MAB6860
Species: Hu
Applications: IP, WB
NBP1-33116
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
AF1905
Species: Mu
Applications: WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
DY262
Species: Hu
Applications: ELISA
348-RB
Species: Hu
Applications: Bioactivity

Publications for ErbB3/Her3 (348-RB)(12)

We have publications tested in 4 confirmed species: Human, Rat, N/A, Yeast.

We have publications tested in 5 applications: Bioassay, Direct ELISA, Dot Blot, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(7)
Direct ELISA
(1)
Dot Blot
(1)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Rat
(2)
N/A
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 348-RB Applications Species
D Gutsch, R Jenke, T Büch, A Aigner Inhibition of HER Receptors Reveals Distinct Mechanisms of Compensatory Upregulation of Other HER Family Members: Basis for Acquired Resistance and for Combination Therapy Cells, 2021;10(2):. 2021 [PMID: 33572976] (Human) Human
A El-Sayed, W Bernhard, K Barreto, C Gonzalez, W Hill, L Pastushok, H Fonge, CR Geyer Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts Theranostics, 2018;8(17):4856-4869. 2018 [PMID: 30279742] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
AA Memon, SC Gilliver, M Borre, J Sundquist, K Sundquist, E Nexo, BS Sorensen Soluble HER3 predicts survival in bladder cancer patients Oncol Lett, 2018;15(2):1783-1788. 2018 [PMID: 29434875] (Bioassay) Bioassay
N Shinozaki, R Hashimoto, K Fukui, S Uchiyama Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities Sci Rep, 2017;7(1):5794. 2017 [PMID: 28725057] (Bioassay, N/A) Bioassay N/A
C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3 Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28507002] (Bioassay, Human) Bioassay Human
Y Miyamoto, T Torii, A Tanoue, K Kawahara, M Arai, H Tsumura, T Ogata, M Nagao, N Terada, M Yamamoto, S Takashima, J Yamauchi Neuregulin-1 type III knockout mice exhibit delayed migration of Schwann cell precursors Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28322798] (Bioassay, Rat) Bioassay Rat
A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (Direct ELISA, Human) Direct ELISA Human
Sachdev S Sidhu A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (Western Blot) Western Blot
Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, Carvalho J, Gaspar M, Weller S, Medcalf M, Wydro M, Pegram R, Mudde G, Bauer A, Moulder K, Woisetschlager M, Tuna M, Haurum J, Sun H A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis. Mol Ther, 2015;23(11):1722-33. 2015 [PMID: 26234505] (Bioassay, Human) Bioassay Human
Xu , Lihui, Kohli , Neeraj, Rennard , Rachel, Jiao , Yang, Razlog , Maja, Zhang , Kathy, Baum , Jason, Johnson , Bryan, Tang , Jian, Schoeberl , Birgit, Fitzgerald , Jonathan, Nielsen , Ulrik, Lugovskoy , Alexey A Rapid optimization and prototyping for therapeutic antibody-like molecules. MAbs, 2013;5(2):237-54. 2013 [PMID: 23392215] (Bioassay, Yeast) Bioassay Yeast
Show All 12 Publications.

Reviews for ErbB3/Her3 (348-RB) (0)

There are no reviews for ErbB3/Her3 (348-RB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ErbB3/Her3 (348-RB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ErbB3/Her3 Products

Bioinformatics Tool for ErbB3/Her3 (348-RB)

Discover related pathways, diseases and genes to ErbB3/Her3 (348-RB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ErbB3/Her3 (348-RB)

Discover more about diseases related to ErbB3/Her3 (348-RB).
 

Pathways for ErbB3/Her3 (348-RB)

View related products by pathway.

PTMs for ErbB3/Her3 (348-RB)

Learn more about PTMs related to ErbB3/Her3 (348-RB).

Blogs on ErbB3/Her3.

Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol ERBB3
Uniprot