Recombinant Human Chondrolectin His-tag Protein, CF

When Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 (875-B5) binds with an ED50 of 5.00󈛂.0 ng/mL.

2 μg/lane of Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Chondrolectin His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Chondrolectin His-tag Protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 binds with an ED₅₀ of 5.00-30.0 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Chondrolectin protein
  Arg22-Asn216, with C-terminal 6-His tag
• Accession #: Q9H9P2.2
• N-terminal Sequence: Arg22
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-40 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1.00 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Chondrolectin His-tag Protein, CF

• C21orf68
• CHODL
• Chondrolectin
• chromosome 21 open reading frame 68
• FLJ12627
• MT75
• PRED12
• Transmembrane protein MT75

Background

Chondrolectin (CHODL), also known as transmembrane protein MT75, is a type I transmembrane protein belonging to the C-type lectin-like domain superfamily. CHODL is predominantly expressed by the vascular muscle of the testis and smooth muscle of the heart and prostate (1) Mature CHODL consists of an extracellular domain (ECD) containing one C-type lectin carbohydrate recognition domain (CRD), a helical transmembrane domain, and a cytoplasmic tail. The ECD of CHODL shares 94% and 95% amino acid sequence identity with mouse and rat CHODL, respectively. Multiple isoforms of CHODL with varying C-terminal deletions are generated as a result of alternative splicing (2,3). CHODL is required for proper neuromuscular junction differentiation and is tightly regulated during early embryonic development (4). Dysregulation of CHODL expression is associated with spinal muscular atrophy in mouse (3). Overexpression of CHODL is associated with enhanced cell growth and invasion in lung cancer, while silencing of CHODL in colorectal cancer is a poor prognostic factor (5,6). Expression of specific CHODL isoforms is associated with T cell maturation (2).

1. Weng, L. et al. (2002) Genomics. 80:62.
2. Weng, L. et al. (2003) J. Biol. Chem. 278:19164.
3. Sleigh, J. et al. (2013) Hum. Mol. Genet. 23:855.
4. Oprişoreanu, A.M. et al. (2019) Cell Rep. 29:1082.
5. Masuda, K. et al. (2011) Clin. Cancer Res. 17:7712.
6. Zhang, X. et al. (2020) J. Cancer. 11:2874.

Bioinformatics

• Uniprot:
  • Q9H9P2.2()