Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF

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Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera (Catalog #9408-B7) inhibits anti-CD3antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is0.6-3 µg/mL.

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.6-3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey VISTA/B7-H5/PD-1H protein
Cynomolgus Monkey VISTA/B7-H5
(Phe33-Ala194)
Accession # XP_005565644
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe33
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
62-71 kDa, reducing conditions
Publications
Read Publication using
9408-B7 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF

  • 4632428N05Rik
  • B7H5
  • B7-H5
  • C10orf54
  • chromosome 10 open reading frame 54
  • Dies1
  • Gi24
  • PD1H
  • PD-1H
  • platelet receptor Gi24
  • PP2135
  • SISP1
  • stress induced secreted protein 1
  • VISTA
  • VSIR

Background

V-domain Ig suppressor of T cell activation (VISTA), also known as platelet receptor Gi24, Dies1, SISP1, PD-1H, and B7‑H5, is a 45-55 kDa transmembrane glycoprotein with homology to B7-like immune co-stimulatory molecules (1, 2). Mature cynomologus VISTA contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, cynomologus VISTA shares 96% and 69% aa sequence identity with human and mouse VISTA, respectively. The 30 kDa ECD can be shed by MT1‑MMP, with a 25-30 kDa fragment remaining in the membrane (3). VISTA promotes both MT1‑MMP expression and the MT1-MMP mediated activation of MMP-2 (3). VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP4 induced signaling in ESC, but is also down regulated following BMP4 exposure (4, 5). It binds to BMP4 directly, and also associates with the type I BMP receptor Activin RIB/ALK 4 (4, 5). VISTA is highly expressed on mature CD11b high myeloid-derived APCs and to a lesser extent on CD4+, CD8+, and T regs and is also found on tumor infiltrating lymphocytes (7). It is up regulated in vivo on activated monocytes and dendritic cells (5). VISTA inhibits CD4+ and CD8+ T cell proliferation, and their production of IL2 and IFN-gamma (6). Its expression on tumor cells attenuates the antitumor immune response and enables more rapid tumor progression (6). In contrast, VISTA limits disease progression in the autoimmune disease model EAE (6). VISTA-Ig suppressed proliferation of T cells but not B cells and blunted the production of T cell cytokines and activation markers, suggesting that VISTA as a negative checkpoint regulator suppresses T cell activation (8, 9).
  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem.285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.
  7. Flies, D.B. et al (2014) J Clin Invest. 124:1966.
  8. Lines, J.L. et al. (2014) Cancer Res. 74:1924.
  9. Nowak, E.C. et al. (2017) Immunol Rev. 276:66.

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Publications for VISTA/B7-H5/PD-1H (9408-B7)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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