Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF

Measured by its binding ability in a functional ELISA. When Recombinant Human EDA-A1/Ectodysplasin A1 Protein (3944-ED) is immobilized at 2.00 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR His-tag Protein (Catalog # 11350-ER) binds with an ED50 of 0.200-3.00 μg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR His-tag Protein (Catalog # 11350-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-41 kDa.

• Reactivity: Pm-Cm, RM
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human EDA-A1/Ectodysplasin A1 Protein (Catalog # 3944-ED) is immobilized at 2.00 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR His-tag (Catalog # 11350-ER) binds with an ED₅₀ of 0.200-3.00 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived EDAR protein
  Glu27-Ala187, with a C-terminal 6-His tag
• Accession #: XP_005575282.1
• N-terminal Sequence: Glu27; determined by Protein ID
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-41 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF

• Anhidrotic ectodysplasin receptor 1
• DLED3
• Ectodermal dysplasia receptor
• ectodysplasin 1, anhidrotic receptor
• ectodysplasin A receptor
• Ectodysplasin-A receptor
• ED1R
• ED5
• EDA1R
• EDA3EDA-A1 receptor
• EDA-A1R
• EDAR
• FLJ94390
• HRM1
• mouse, homolog of
• tumor necrosis factor receptor superfamily member EDAR

Background

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Based on its high homology with human EDAR, cynomologus EDAR is predicted to be a 488 amino acid (aa) protein with a 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The cynomolgus and human EDAR homologs share 99% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

1. Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
4. Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
6. Yan, M. et al. (2000) Science 290:523.

Bioinformatics

• Uniprot:
  • XP_005575282.1()