Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Recombinant Cynomolgus Monkey R-Spondin 3 (Catalog # 10948-RS) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4‑10 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey R-Spondin 3 Protein (Catalog # 10948-RS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-44 kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF Summary

• Details of Functionality: Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.4-10 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey R-Spondin 3 protein
  Met33-His273
• Accession #: XP_005551811.1
• N-terminal Sequence: Met33
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-44 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

• Cristin 1
• CRISTIN1
• FLJ14440
• hPWTSR
• hRspo3
• Protein with TSP type-1 repeat
• PWTSR
• Roof plate-specific spondin-3
• RSPO3
• R-spondin 3 homolog (Xenopus laevis)
• RSpondin 3
• R-Spondin 3
• R-spondin-3
• Thrombospondin type-1 domain-containing protein 2
• thrombospondin, type I, domain containing 2
• THSD2

Background

Roof plate-specific spondin 3 (RSPO3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin1), is member of the R-Spondin family of proteins which are involved in the activation and regulation of Wnt signaling pathway. The R-Spondin family, consisting of 4 members, are extracellular glycoproteins which share ~60% amino acid (aa) homology and a conserved protein structure (1). Similar to other R-Spondins, RSPO3 contains two adjacent cysteine-rich furin-like domains with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif and a C-terminal tail rich in basic residues. The furin-like domains are needed for beta -catenin stabilization (2, 3). Cynomologus RSPO3 shares 97% and 88% aa sequence identity with human and mouse RSPO3, respectively. While every member of the R-Spondin family positive modulators of Wnt/ beta -catenin signaling, each one has a distinct expression pattern (1 - 5). In mouse, RSPO3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (5, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (10). RSPO3 has also been identified as an oncogene (11).

1. Kim, K.-A. et al. (2006) Cell Cycle, 5:23.
2. Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
3. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
4. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
5. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
6. Kazanskaya, O. et al. (2008) Development 135:3655.
7. Aoki, M. et al. (2007) Dev. Biol. 301:218.
8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.

Bioinformatics

• Uniprot:
  • XP_005551811.1()