Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

When Recombinant Human R-Spondin 3 Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) iscoated at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48Fc Chimera (Catalog # 10112-GP) binds with an ED50 of 6-36 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 100-111 kDa and 200-220 kDa, respectively.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera binds with an ED₅₀ of 6-36 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Lgr4/GPR48 protein
  

  Cynomolgus Monkey Lgr4 (Ala25-Thr544) Accession # NP_001252594	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001252594
• N-terminal Sequence: Ala25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-111 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

• GPR48
• GPR48G protein-coupled receptor 48
• G-protein coupled receptor 48
• leucine-rich repeat containing G protein-coupled receptor 4
• Lgr4

Background

Lgr4 (leucine‑rich repeat GPR 4), also called GPR48 (G‑protein‑coupled receptor 48), is a seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (1‑6). Lgr4 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors (5). It acts at least in part by enhancing Wnt‑dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta ‑catenin (3, 4). Cynomolgus Monkey Lgr4 cDNA encodes 951 amino acids (aa), including a long N‑terminal extracellular domain with multiple LRR domains that may mediate ligand interaction. The LRR‑containing ECD of Cynomolgus Monkey Lgr4 shares 99% aa sequence identity with human Lgr4. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7‑9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over‑expressed in carcinomas and may promote invasiveness and metastasis by down‑regulating p27Kip1 expression (10).

1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
5. de Lau, W. et al. (2011) Nature 476:293.
6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.
10. Gao, Y. et al. (2006) Cancer Res. 66:11623.

Bioinformatics

• Uniprot:
  • NP_001252594()