RC74 Antibody - BSA Free

Western Blot: RC74 Antibody - BSA Free [NBP3-46468] - Jurkat cells were subjected to SDS PAGE followed by western blot with (RC74 antibody) at dilution of 1:1000

Immunoprecipitation: RC74 Antibody - BSA Free [NBP3-46468] - IP Result of anti-RC74 (IP:, 4ug; Detection: 1:1000) with HeLa cells lysate 880ug.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RC74 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RC74 Antibody - BSA Free (NBP3-46468) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: integrator complex subunit 9. (Uniprot# Q9NV88)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INTS9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:200-1:2000
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RC74 Antibody - BSA Free

• CPSF2L
• FLJ10871
• INT9
• integrator complex subunit 9
• Protein related to CPSF subunits of 74 kDa
• RC74
• RC-74

Background

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INTS9