RBCK1 Overexpression Lysate


Western Blot: RBCK1 Overexpression Lysate (Adult Normal) [NBL1-15187] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBCK1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

RBCK1 Overexpression Lysate Summary


RBCK1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC203906

Accession#: NM_031229

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
57.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RBCK1 Overexpression Lysate

  • C20orf18
  • EC 6.3.2.-
  • HBV associated factor 4
  • HBV-associated factor 4
  • Heme-oxidized IRP2 ubiquitin ligase 1
  • Hepatitis B virus X-associated protein 4
  • HOIL-1
  • RanBP-type and C3HC4-type zinc finger containing 1
  • ranBP-type and C3HC4-type zinc finger-containing protein 1
  • RBCC protein interacting with PKC1
  • RBCK2
  • RNF54RING finger protein 54
  • ubiquitin conjugating enzyme 7 interacting protein 3
  • Ubiquitin-conjugating enzyme 7-interacting protein 3
  • XAP3
  • XAP4chromosome 20 open reading frame 18
  • ZRANB4


The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC, Simple Western, WB
Species: Hu, Mu, Rt
Applications: B/N, Func-Inh, In vitro, In vivo
Species: Hu, Mu
Applications: ICC/IF, IHC, IP, WB
Species: Hu
Applications: ELISA, ICC/IF, PLA, S-ELISA, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: GS, GS, ICC/IF, IHC, IHC-P, WB
Species: Hu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ELISA, WB
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu
Applications: ICC/IF, KD, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu
Applications: IHC, IHC-P, WB

Publications for RBCK1 Lysate (NBL1-15187) (0)

There are no publications for RBCK1 Lysate (NBL1-15187).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RBCK1 Lysate (NBL1-15187) (0)

There are no reviews for RBCK1 Lysate (NBL1-15187). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RBCK1 Lysate (NBL1-15187) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RBCK1 Products

Diseases for RBCK1 Lysate (NBL1-15187)

Discover more about diseases related to RBCK1 Lysate (NBL1-15187).

Pathways for RBCK1 Lysate (NBL1-15187)

View related products by pathway.

PTMs for RBCK1 Lysate (NBL1-15187)

Learn more about PTMs related to RBCK1 Lysate (NBL1-15187).

Research Areas for RBCK1 Lysate (NBL1-15187)

Find related products by research area.

Blogs on RBCK1

There are no specific blogs for RBCK1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

RBCK1 Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our RBCK1 Overexpression Lysate and receive a gift card or discount.


Gene Symbol RBCK1