RBBP4/RbAp48 Antibody (11G10) [Alexa Fluor® 647]

Product Discontinued

RBBP4/RbAp48 Antibody (11G10) [Alexa Fluor® 647] Summary

• Immunogen: The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli.
• Specificity: This is specific for full length RbAp48.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBBP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RBBP4/RbAp48 Antibody (11G10) [Alexa Fluor® 647]

• CAF-1 p48
• CAF-1 subunit C
• CAF-1C
• CAF-I p48
• Chromatin assembly factor 1 subunit C
• Chromatin assembly factor I p48 subunit
• chromatin assembly factor/CAF-1 p48 subunit
• histone-binding protein RBBP4
• MSI1 protein homolog
• Nucleosome-remodeling factor subunit RBAP48
• NURF55
• RBAP48
• RBBP4
• RBBP-4
• retinoblastoma binding protein 4
• Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
• Retinoblastoma-binding protein p48

Background

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP4