RASA4 Antibody

Product Discontinued

RASA4 Antibody Summary

• Immunogen: Synthetic peptide, AKRSSLYIRIVEGC, representing the N Terminus of the human protein according to NP_008920.4
• Specificity: This antibody is expected to recognise both reported isoforms (NP_008920.5; NP_001073346.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:1000
• Application Notes: Preliminary experiments gave an approx 70kDa band in Human Liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 90.4kDa according to NP_008920.4. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RASA4 Antibody

• Ca2+-promoted Ras inactivator
• Calcium-promoted Ras inactivator
• CAPRIGAPLKIAA0538RASA4B
• FLJ59070
• Putative Ras GTPase-activating protein 4B
• ras GTPase-activating protein 4
• RAS p21 protein activator 4MGC131890
• RasGAP-activating-like protein 2

Background

The RASA4 gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this prote

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RASA4
• Entrez:
  • 10156(Human)
• Uniprot:
  • NP_008920.4(Human)