RAR alpha/NR1B1 Antibody

Western Blot: RAR alpha/NR1B1 Antibody [NB300-969] - Staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RAR alpha/NR1B1 Antibody Summary

• Description: Novus Biologicals Goat RAR alpha/NR1B1 Antibody (NB300-969) is a polyclonal antibody validated for use in WB and ELISA. Anti-RAR alpha/NR1B1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SPSLSPSSHRSSPATQSP corresponding to C-Terminus according to NP_000955.1, NP_001138773.1, NP_001019980.1, NP_001138774.1.
• Specificity: This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RARA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:8000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx 55kDa band observed in Human Breast and Breast cancer lysates (calculated MW of 50.8kDa according to NP_000955.1and NP_001019980.1).
• Control:

  RAR alpha/NR1B1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAR alpha/NR1B1 Antibody

• alpha polypeptide
• NR1B1
• RAR alpha
• RARA
• RAR-alpha
• retinoic acid receptor, alpha

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAR alpha/NR1B1 Antibody (NB300-969)(1)

Publication using NB300-969

• Farias EF, Ong DE, Ghyselinck NB et al. Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity. J Natl Cancer Inst 2005-01-05 [PMID: 15632377]

Bioinformatics

• Gene Symbol: RARA
• Entrez:
  • 5914(Human)
• Uniprot:
  • NP_000955.1(Human)