RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

Western Blot: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Analysis of RARA expression in Hela S3 NE (Cat # L013V3).

Immunohistochemistry-Paraffin: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Analysis of monoclonal antibody to RARA on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1.2 ug/ml

ELISA: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Detection limit for recombinant GST tagged RARA is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RARA (NP_000955, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLS
• Specificity: RARA (2D2)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RARA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

• alpha polypeptide
• NR1B1
• RAR alpha
• RARA
• RAR-alpha
• retinoic acid receptor, alpha

Background

Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAR alpha/NR1B1 Antibody (H00005914-M03)(1)

Publication using H00005914-M03

• Voss AK, Collin C, Dixon MP et al. Moz and Retinoic Acid Coordinately Regulate H3K9 Acetylation, Hox Gene Expression, and Segment Identity. Dev Cell 2009-11-01 [PMID: 19922872]

Bioinformatics

• Gene Symbol: RARA
• Entrez:
  • 5914(Human)
• OMIM:
  • 180240(Human)
• Uniprot:
  • NP_000955(Human)