RAR alpha/NR1B1 Antibody (1C10) - Azide and BSA Free

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - RARA monoclonal antibody (M02), clone 1C10. Analysis of RARA expression in NIH/3T3.

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - RARA monoclonal antibody (M02), clone 1C10 Analysis of RARA expression in Hela S3 NE.

Proximity Ligation Assay: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - Analysis of protein-protein interactions between RXRA and RARA. HeLa cells were stained with anti-RXRA rabbit purified polyclonal 1:1200 and anti-RARA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Func
• Clone: 1C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAR alpha/NR1B1 Antibody (1C10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RARA (NP_000955, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLS
• Localization: Nuclear
• Specificity: RARA - retinoic acid receptor, alpha
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RARA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAR alpha/NR1B1 Antibody (1C10) - Azide and BSA Free

• alpha polypeptide
• NR1B1
• RAR alpha
• RARA
• RAR-alpha
• retinoic acid receptor, alpha

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RARA
• Entrez:
  • 5914(Human)
• OMIM:
  • 180240(Human)
• Uniprot:
  • NP_000955(Human)