RAP30 Antibody

Western Blot: RAP30 Antibody [NBP1-90812] - Analysis in control (vector only transfected HEK293T lysate) and GTF2F1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: RAP30 Antibody [NBP1-90812] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cell junctions.

Immunohistochemistry-Paraffin: RAP30 Antibody [NBP1-90812] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Product Discontinued

RAP30 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQD
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2F1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RAP30 Antibody

• ATP-dependent helicase GTF2F2
• BTF4
• General transcription factor IIF 30 kDa subunit
• general transcription factor IIF subunit 2
• general transcription factor IIF, polypeptide 2, 30kDa
• TF2F2
• TFIIF
• TFIIF-beta
• Transcription initiation factor IIF subunit beta
• Transcription initiation factor RAP30

Background

Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2F1