RAMP2 Antibody [Alexa Fluor® 647]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

RAMP2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human RAMP2
  Gln43-Val145
  Accession # O60895
• Specificity: Detects human RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse RAMP2 is observed and less than 1% cross-reactivity with recombinant human (rh) RAMP1 and rhRAMP3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human RAMP2 (receptor activity modifying protein 2) is a 17 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature human RAMP2 is a 133 amino acid (aa) type I transmembrane glycoprotein that contains a 103 aa extracellular domain (ECD) (aa 43-145) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 86-92 are critical for AM binding. There is one potential splice variant that shows a five aa insertion after Glu54. Over aa 43-145, human RAMP2 shares 61% aa identity with mouse RAMP2. Members of RAMP family of proteins are known to form complexes with apparent molecular weight of 25 kDa to 50 kDa that are resistant to denaturing and reducing agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.