RAMP1 Antibody

Western Blot: RAMP1 Antibody [NBP1-20966] - (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RAMP1 Antibody Summary

• Immunogen: Peptide with sequence C-DYQSKRTEGIV corresponding to C-Terminus according to NP_848488.2.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize isoform 1 (NP_058590.1) only.
• Predicted Species: Human (100%), Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAMP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 18 kDa band and 40 kDa band in rat brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in scientific literature with different antibodies (PMID: 11535606). This protein has a calculated MW of 16.9 kDa band according to mouse NP_058590.1 and 16.8 kDa band according to rat NP_113833.1.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAMP1 Antibody

• calcitonin receptor-like receptor activity modifying protein 1
• Calcitonin-receptor-like receptor activity-modifying protein 1
• CRLR activity-modifying protein 1
• RAMP1
• receptor (calcitonin) activity modifying protein 1
• receptor (G protein-coupled) activity modifying protein 1
• receptor activity modifying protein 1
• receptor activity-modifying protein 1

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAMP1 Antibody (NBP1-20966)(4)

Publications using NBP1-20966

• Guo TZ, Wei T, Li WW et al. Immobilization contributes to exaggerated neuropeptide signaling, inflammatory changes, and nociceptive sensitization after fracture in rats. J Pain 2014-07-22 [PMID: 25063543] (WB, Rat)
  
  Details:
  RAMP1 antibody used for WB in hind paw skin of Rats after 4 weeks of cast immobilization of a tibia fracture (Fx+cast), cast immobilization alone (Cast) or after fracture with intramedullary pinning with early mobilization (Fx+pin) - Figure 2C
• Shi X, Wang L, Clark JD, Kingery WS. Keratinocytes express cytokines and nerve growth factor in response to neuropeptide activation of the ERK1/2 and JNK MAPK transcription pathways. Regul Pept. 2013-08-17 [PMID: 23958840] (WB, Rat)
• Tsujikawa K, Yayama K, Hayashi T et al. Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice. Proc Natl Acad Sci U S A 2007-10-16 [PMID: 17923674]
• Hilairet S, Belanger C, BertrJ et al. Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin. J Biol Chem 2001-11-01 [PMID: 11535606]

Bioinformatics

• Gene Symbol: RAMP1
• Entrez:
  • 10267(Human)
  • 51801(Mouse)
  • 58965(Rat)
• Uniprot:
  • NP_058590.1(Mouse)