RAE1 Antibody

Western Blot: RAE1 Antibody [NBP2-80421] - Analysis of RAE1 on different lysates using anti-RAE1 antibody at 1/500 dilution. Positive control: Lane 1: PC-3 Lane 2: Mouse testis tissue Lane 3: Rat testis tissue

Immunocytochemistry/Immunofluorescence: RAE1 Antibody [NBP2-80421] - Staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Flow Cytometry: RAE1 Antibody [NBP2-80421] - Analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RAE1 Antibody [NBP2-80421] - Staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RAE1 Antibody [NBP2-80421] - Staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Product Discontinued

RAE1 Antibody Summary

• Immunogen: Synthetic peptide within Human RAE1 aa 319-368 / 368. (SwissProt: P78406 Human; SwissProt: Q8C570 Mouse; SwissProt: Q3SWS8 Rat)
• Localization: Nucleus. Cytoskeleton.Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAE1 Antibody

• dJ481F12.3
• dJ800J21.1
• FLJ30608
• homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer)
• MGC117333
• MGC126076
• MGC126077
• MIG14
• migration-inducing gene 14
• Mnrp41
• mRNA export factor
• mRNA export protein
• mRNA-associated protein mrnp 41
• mRNA-binding protein, 41-kD
• MRNP41
• RAE1 (RNA export 1, S.pombe) homolog
• Rae1 protein homolog
• RAE1 RNA export 1 homolog (S. pombe)

Background

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAE1