Recombinant Human RAB32 Protein

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SDS-Page: Recombinant Human RAB32 Protein [NBP1-72487] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human RAB32 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-225 of Human RAB32

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RAB32
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RAB32 Protein

  • RAB32, member RAS oncogene family
  • ras-related protein Rab-32

Background

RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1. Through a combination of its functions as a PKA-anchoring protein and a regulator of MAM properties, the activity and expression level of RAB32 determine the speed of apoptosis onset. Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RAB32