Pyruvate Dehydrogenase Kinase 1/PDK1 [p Ser241] Antibody (EPR336(2))

Product Discontinued

Pyruvate Dehydrogenase Kinase 1/PDK1 [p Ser241] Antibody (EPR336(2)) Summary

• Immunogen: A phospho specific peptide corresponding to residues surrounding Serine 241 of human PDK1 was used as an immunogen.
• Modification: p Ser241
• Specificity: This antibody only detects PDK1 phosphorylated at Serine 241.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDK1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:10-100
  • Western Blot 1:1000-10000

Reactivity Notes

Reacts with: Mouse, Rat, Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Pyruvate Dehydrogenase Kinase 1/PDK1 [p Ser241] Antibody (EPR336(2))

• EC 2.7.11
• EC 2.7.11.2
• mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• PDH kinase 1
• PDK1
• PDK-1
• PKB kinase
• Pyruvate dehydrogenase kinase isoform 1
• pyruvate dehydrogenase kinase, isoenzyme 1
• pyruvate dehydrogenase kinase, isozyme 1
• Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDK-1 Antibody (NBP1-96065)(1)

Publication using NBP1-96065

• Young Vicky J, Brown Jeremy K, Maybin Jacqueline et al. Transforming growth factor-beta induced Warburg-like metabolic reprogramming may underpin the development of peritoneal endometriosis. J Clin Endocrinol Metab. 2014-05-05 [PMID: 24796928] (Human)

Bioinformatics

• Gene Symbol: PDK1
• Uniprot:
  • O15530()