PYK2/FAK2 Antibody

Western Blot: PYK2/FAK2 Antibody [H00002185-D01P] - Analysis of PTK2B expression in rat brain.

Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [H00002185-D01P] - Analysis of antibody to PTK2B on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: PYK2/FAK2 Antibody [H00002185-D01P] - Analysis of PTK2B expression in transfected 293T cell line by PTK2B polyclonal antibody.Lane 1: PTK2B transfected lysate(115.90 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: PYK2/FAK2 Antibody [H00002185-D01P] - Analysis of protein-protein interactions between PTK2B and ERBB2. HeLa cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-ERBB2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PYK2/FAK2 Antibody [H00002185-D01P] - Analysis of protein-protein interactions between PTK2B and YES1. Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

PYK2/FAK2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PTK2B (AAH36651.1, 1 a.a. - 1009 a.a.) full-length human protein. MSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIREIITSILLSGRIGPNIRLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDAKPTCLAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDGYCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELYPYGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAMDAKNLLDAVDQAKVLANLAHPPAE
• Specificity: PTK2B - PTK2B protein tyrosine kinase 2 beta,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTK2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PYK2/FAK2 Antibody

• CADTK
• CADTKCAK-beta
• CAK beta
• CAKB
• Calcium-dependent tyrosine kinase
• Cell adhesion kinase beta
• EC 2.7.10
• FADK2
• FAK2
• FAK2EC 2.7.10.2
• Focal adhesion kinase 2
• FRNK
• PKB
• ppyk2
• Proline-rich tyrosine kinase 2
• protein kinase B
• protein tyrosine kinase 2 beta
• protein-tyrosine kinase 2-beta
• PTK
• PTK2B protein tyrosine kinase 2 beta
• PTK2B
• PYK2
• PYK2Related adhesion focal tyrosine kinase
• RAFTK
• RAFTKFADK 2

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTK2B
• Entrez:
  • 2185(Human)
• Uniprot:
  • AAH36651.1(Human)