PYK2/FAK2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PYK2/FAK2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PYK2
  Asn221-Arg411
  Accession # Q14289
• Specificity: Detects endogenous human PYK2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PYK2/FAK2 Antibody [Alexa Fluor® 488]

• CADTK
• CADTKCAK-beta
• CAK beta
• CAKB
• Calcium-dependent tyrosine kinase
• Cell adhesion kinase beta
• EC 2.7.10
• FADK2
• FAK2
• FAK2EC 2.7.10.2
• Focal adhesion kinase 2
• FRNK
• PKB
• ppyk2
• Proline-rich tyrosine kinase 2
• protein kinase B
• protein tyrosine kinase 2 beta
• protein-tyrosine kinase 2-beta
• PTK
• PTK2B protein tyrosine kinase 2 beta
• PTK2B
• PYK2
• PYK2Related adhesion focal tyrosine kinase
• RAFTK
• RAFTKFADK 2

Background

PYK2 (Proline-rich tyrosine kinase 2; also FAK2, RAFTK and CAK beta ) is a 112‑116 kDa member of the FAK subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. It contains one FERM domain that binds to growth factor receptors (aa 39‑359), a protein kinase domain (aa 425‑683), two Pro‑rich segments that bind SH domains (aa 702‑767 and 831‑869), and one FAT region that interacts with integrins (aa 868‑1009). Phosphorylation of PYK2 at Tyr402/579/580 is associated with changes in activity. There is one splice variant that shows a deletion of aa 739‑780.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.