PTH1R/PTHR1 Antibody (4D2)

Images

 
Western Blot: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis using PTH1R mAb against PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of human kidney tissues using PTH1R mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PTH1R/PTHR1 Antibody (4D2) Summary

Immunogen
Purified recombinant fragment of human PTH1R expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTH1R
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PTH1R/PTHR1 Antibody (4D2)

  • MGC138426
  • parathyroid hormone 1 receptorMGC138452
  • parathyroid hormone/parathyroid hormone-related peptide receptor
  • parathyroid hormone/parathyroid hormone-related protein receptor
  • PFE
  • PTH/PTHr receptor
  • PTH/PTHrP type I receptor
  • PTH1 receptor
  • PTH1R
  • PTHR
  • PTHR1
  • PTHRPTHR1parathyroid hormone receptor 1
  • seven transmembrane helix receptor

Background

PTHR1, a Parathyroid Hormone Receptor, mediates PTH-dependent regulation of mineral-ion homeostasis. It also mediates the paracrine action of PTHRP in endochondral bone formation. Mutations in this receptor are responsible for disorders of calcium and bone metabolism such as chondrodysplasia, hypercalcemia, and chronic renal failure. Three promoters, P1, P2, and P3, regulate the expression of PTHR. PTHR1 has been reported to be expressed in bone, breast, brain, colon, GI, kidney, liver, placenta, skin, umbilical cord, and uterus. ESTs have been isolated from B-cell/lung/testis, brain, breast, fetus, heart, heart/melanocyte/uterus, kidney, liver/spleen, lung, placenta, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTH1R
Entrez
Uniprot