PTGER4/EP4 Antibody

Western Blot: PTGER4/EP4 Antibody [NB100-2801] - (0.1ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PTGER4/EP4 Antibody Summary

• Description: Novus Biologicals Goat PTGER4/EP4 Antibody (NB100-2801) is a polyclonal antibody validated for use in WB and ELISA. Anti-PTGER4/EP4 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LEREVSKNPDLQA corresponding to internal region according to NP_000949.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTGER4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human testis lysates (calculated MW of 53.1 kDa band according to NP_000949.1).
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25894216).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTGER4/EP4 Antibody

• EP4
• EP4R
• MGC126583
• PGE receptor EP4 subtype
• PGE receptor, EP4 subtype
• PGE2 receptor EP4 subtype
• prostaglandin E receptor 4 (subtype EP4)
• prostaglandin E2 receptor EP4 subtype
• Prostanoid EP4 receptor
• PTGER2
• PTGER4

Background

Prostaglandin E receptor EP4 is a Prostanoid Receptor with a relaxing effect on smooth muscle. It may play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. EP4 has been reported to be expressed in blood, colon, immune tissue, intestine, kidney, lung, ovary, skin, and uterus. ESTs have been isolated from a wide range of human tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTGER4/EP4 Antibody (NB100-2801)(3)

Publications using NB100-2801

• Parida S, Pal I, Parekh A et al. GW627368X inhibits proliferation and induces apoptosis in cervical cancer by interfering with EP4/EGFR interactive signaling. Cell Death Dis. 2016-03-25 [PMID: 27010855] (WB, Human)
• Parida S, Parekh A, Dey G et al. Molecular Inhibition of Prostaglandin E2 with GW627368X: Therapeutic potential and Preclinical Safety Assessment in Mouse Sarcoma Model Cancer Biol. Ther. 2015-04-20 [PMID: 25894216] (WB, Mouse)
• Echeverria V, Clerman A, Dore S. Stimulation of PGE receptors EP2 and EP4 protects cultured neurons against oxidative stress and cell death following beta-amyloid exposure. Eur J Neurosci 2005-11-01 [PMID: 16262658]

Bioinformatics

• Gene Symbol: PTGER4
• Entrez:
  • 5734(Human)
• Uniprot:
  • NP_000949.1(Human)