Rat PTGER2 ELISA Kit (Colorimetric)

ELISA: Rat PTGER2 ELISA Kit (Colorimetric) [NBP3-00461] - Samples were spiked with high concentrations of Rat PTGER2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat PTGER2 ELISA Kit (Colorimetric) [NBP3-00461] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 39.06 - 2500 pg/mL
• Sensitivity: 23.44 pg/mL

Rat PTGER2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat PTGER2 in samples. No significant cross-reactivity or interference between Rat PTGER2 and analogues was observed.
• Standard Curve Range: 39.06 - 2500 pg/mL
• Sensitivity: 23.44 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 8.86%
• Intra-Assay: 8.96%
• Spike Recovery: 88 - 111%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PTGER2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat PTGER2 ELISA Kit (Colorimetric)

• EP2
• PGE receptor EP2 subtype
• PGE2 receptor EP2 subtype
• prostaglandin E receptor 2 (subtype EP2), 53kD
• prostaglandin E receptor 2 (subtype EP2), 53kDa
• prostaglandin E2 receptor EP2 subtype
• Prostanoid EP2 receptor
• PTGER2

Background

Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism.Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors,designated EP1, EP2, EP3 and EP4. The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney. EP1,which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers. EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase. EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion. EP3 is expressed in renal vessels, thick ascending limb and collecting duct. EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. In uterus, EP3 induces the contraction of uterine smooth muscles.EP4 is expressed in glomerulus and collecting duct. It couples to Gs proteins,which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for PTGER2 ELISA Kit (NBP3-00461)(1)

Publication using NBP3-00461

• Song CX, Liu SY, Zhu WT et al. Excessive mechanical stretch mediated osteoblasts promote the catabolism and apoptosis of chondrocytes via the Wnt/beta catenin signaling pathway Molecular medicine reports 2021-08-01 [PMID: 34165157] (ELISA)

FAQs for PTGER2 ELISA Kit (NBP3-00461). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PTGER2