| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21756-50ul | |||
CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PTGER2 was detected in immersion fixed paraffin-embedded sections of human bladder using Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6656-SP | |||
![Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02321] - Staining of human kidney formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02321-img0003.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02321] - Staining of human kidney formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.")
![Western Blot: PTGER2 Antibody (2G10L6) [NBP3-16740] - Analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Western-Blot-NBP3-16740-img0005.jpg "Western Blot: PTGER2 Antibody (2G10L6) [NBP3-16740] - Analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of U-2 OS cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0004.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody (2G10L6) [NBP3-16740] - Human placenta using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunohistochemistry-Paraffin-NBP3-16740-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of C6 cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of NIH-3T3 cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0003.jpg)
![Western Blot: PTGER2 Antibody (JE30-72) [NBP3-32874] - Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. Lane 1: JAR cell lysate (15 ug/Lane) Lane 2: NIH/3T3 cell lysate (15 ug/Lane) Lane 3: Human placenta tissue lysate (30 ug/Lane) Lane 4: Mouse placenta tissue lysate (30 ug/Lane) Lane 5: Mouse lung tissue lysate (30 ug/Lane) Lane 6: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32874) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-288202412442965.jpg "Western Blot: PTGER2 Antibody (JE30-72) [NBP3-32874] - Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. Lane 1: JAR cell lysate (15 ug/Lane) Lane 2: NIH/3T3 cell lysate (15 ug/Lane) Lane 3: Human placenta tissue lysate (30 ug/Lane) Lane 4: Mouse placenta tissue lysate (30 ug/Lane) Lane 5: Mouse lung tissue lysate (30 ug/Lane) Lane 6: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32874) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTGER2 Antibody (JE30-72) [NBP3-32874] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32874) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-298202414262611.jpg)
![Immunocytochemistry/ Immunofluorescence: PTGER2 Antibody (JE30-72) [NBP3-32874] - Immunocytochemistry analysis of JAR cells labeling PTGER2 with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-29202410484830.jpg)
![Flow Cytometry: PTGER2 Antibody (JE30-72) [NBP3-32874] - Flow cytometric analysis of JAR cells labeling PTGER2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32874, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-29202416453292.jpg)
![Western Blot: PTGER2 Antibody (SR1927) [NBP3-21756] - Western blot analysis using NBP3-21756 in JAR cell lysate.](http://images.novusbio.com/fullsize/nbp3-21756_rabbit-ptger2-mab-sr1927-57202317161828.jpg "Western Blot: PTGER2 Antibody (SR1927) [NBP3-21756] - Western blot analysis using NBP3-21756 in JAR cell lysate.")
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Anti-PTGER2 / EP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0003.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Anti-PTGER2 / EP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - IHC analysis of Ovary, Epithelium using NLS971 at 6 ug/ ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0001.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Analysis of anti-EP2 antibody with human lung.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0002.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02375] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02375-img0002.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02375] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.")
![Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PTGER2-Antibody-8D8-Immunohistochemistry-NBP3-15217-img0001.jpg "Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PTGER2-Antibody-8D8-Immunohistochemistry-NBP3-15217-img0002.jpg)
HAF007). A specific band was detected for PTGER2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using 
