PSMB7 Antibody

Western Blot: Proteasome 20S beta 7 Antibody [NBP1-52081] - NBP1-52081 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PSMB7 Antibody Summary

• Description: Novus Biologicals Goat PSMB7 Antibody (NBP1-52081) is a polyclonal antibody validated for use in WB and ELISA. Anti-PSMB7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RNAVLEADFAKR corresponding to internal region according to NP_002790.1.
• Epitope: C-RNAVLEADFAKR
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMB7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in lysates of HEK 293, HeLa and Jurkat and of mouse Fetal brain (calculated MW of 30.0 kDa band according to NP_002790.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSMB7 Antibody

• Macropain Chain Z
• Proteasome Subunit Z
• PSMB7
• Z

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMB7 Antibody (NBP1-52081)(1)

Publication using NBP1-52081

• Munkacsy G, Abdul-Ghani R, Mihaly Z et al. PSMB7 is associated with anthracycline resistance and is a prognostic biomarker in breast cancer. Br J Cancer 2010-01-19 [PMID: 20010949]

Bioinformatics

• Gene Symbol: PSMB7
• Entrez:
  • 5695(Human)
  • 19177(Mouse)
  • 85492(Rat)
• Uniprot:
  • NP_002790.1(Human)