PSGR Antibody - BSA Free

Immunohistochemistry: PSGR Antibody [NLS6332] - Expression of PSGR protein in normal prostate (N), prostatic intraepithelial neoplasia (PIN) and prostate cancer (PCa) by immunohistochemistry. Low (a and b) and high (c and d) magnification images of PSGR in N and adjacent PIN (a, c), and PIN and adjacent PCa (b, d) (original magnification x 10 and x 20 for low and high respectively). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1921-6), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: PSGR Antibody [NLS6332] - Human Skin, Melanoma tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: PSGR Antibody [NLS6332] - IHC staining of human prostate, epithelium.

Immunohistochemistry-Paraffin: PSGR Antibody [NLS6332] - Skin, Malignant Melanoma

Immunohistochemistry-Paraffin: PSGR Antibody [NLS6332] - Prostate carcinoma

Immunohistochemistry: PSGR Antibody [NLS6332] - Expression of PSGR protein in normal prostate (N), prostatic intraepithelial neoplasia (PIN) and prostate cancer (PCa) by immunohistochemistry. Low (a and b) and high (c and d) magnification images of PSGR in N and adjacent PIN (a, c), and PIN and adjacent PCa (b, d) (original magnification x 10 and x 20 for low and high respectively). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1921-6), licensed under a CC-BY license.

Product Discontinued

PSGR Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 14 amino acid peptide from N-terminal extracellular domain of human PSGR.
• Epitope: N-Terminus extracellular domain
• Specificity: Human OR51E2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OR51E2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 16 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Galago (100%), Marmoset (100%), Equine (86%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSGR Antibody - BSA Free

• HPRAJ
• Olfactory Receptor OR11-16
• olfactory receptor, family 51, subfamily E, member 2
• olfactory receptor, family 51, subfamily E, member 3 pseudogene
• olfactory receptor, family 52, subfamily A, member 2
• OR51E2
• OR51E3P
• OR52A2
• prostate specific G-protein coupled receptor
• Prostate-specific G-protein coupled receptor
• PSGR
• PSGRolfactory receptor 51E2

Background

Prostate-specific G protein-coupled receptor possesses homology to the Olfactory Receptor family. It is expressed specifically in normal prostate, and its expression is upregulated in prostate carcinoma. Prostate-specific G protein-coupled receptor has been shown to be expressed primarily in normal prostate and to be overexpressed in prostate carcinoma. In humans, it has also been shown to be expressed in the olfactory epithelium and, to a lesser extent, in the medulla of the brain. ESTs have been isolated from prostate libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSGR Antibody (NLS6332)(1)

Publication using NLS6332

• Cao W, Li F, Yao J, Yu J. Prostate specific G protein coupled receptor is associated with prostate cancer prognosis and affects cancer cell proliferation and invasion. BMC Cancer. 2015-11-20 [PMID: 26582057] (IHC-P, Human)
  
  Details:
  PSGR antibody was used for IHC-P analysis of human prostate cancer tissue (benign prostatic tissue, high grade prostatic intraepithelial neoplasia, or prostatic adenocarcinoma) microarray sections. The IHC protocol implicated antigen retrieval with EDTA buffer pH 9.0 at 98C for 20 minutes, blocking of endogenous peroxidase with 3% hydrogen peroxide, use of primary at 1: 100 dilution with 1 hour RT incubation, detection using anti-rabbit biotin EnVision?+?System horseradish peroxidase-labeled polymer and DAB followed by counterstaining with Mayer’s hematoxylin. PSGR staining was located in the cytoplasm and cell membranes, and a higher PSGR expression was found in PIN compared to normal tissues.

Bioinformatics

• Gene Symbol: OR51E2
• Entrez:
  • 81285(Human)
• Uniprot:
  • Q9H255()