Protein Phosphatase inhibitor 1 Antibody (SN0756)

Western Blot: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Staining PPP1R1A in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Analysis of paraffin-embedded mouse kidney tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin.

Flow Cytometry: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Flow cytometric analysis of SH-SY5Y cells with Protein Phosphatase inhibitor 1 antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Staining PPP1R1A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Staining PPP1R1A in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Analysis of paraffin-embedded human kidney tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Protein Phosphatase inhibitor 1 Antibody (SN0756) [NBP2-67695] - Analysis of paraffin-embedded mouse brain tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin.

Product Discontinued

Protein Phosphatase inhibitor 1 Antibody (SN0756) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Protein Phosphatase inhibitor 1 aa 1-50 / 171. (SwissProt: Q13522 Human; SwissProt: Q9ERT9 Mouse; SwissProt: P19103 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPP1R1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Protein Phosphatase inhibitor 1 Antibody (SN0756)

• I1
• I-1
• IPP-1
• IPP1inhibitor-1
• protein phosphatase 1 regulatory subunit 1A
• protein phosphatase 1, regulatory (inhibitor) subunit 1A
• Protein phosphatase inhibitor 1
• protein phosphatase inhibitor-1

Background

The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R1A