Protein phosphatase 1F Overexpression Lysate (Native)

Images

 
Western Blot: Protein phosphatase 1F Overexpression Lysate (Adult Normal) [NBL1-14661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein phosphatase 1F.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Protein phosphatase 1F Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210043 and based on accession number NM_014634. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein phosphatase 1F (PP2C domain containing) (PPM1F), mRNA.
Gene
PPM1F

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Protein phosphatase 1F

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Protein phosphatase 1F Overexpression Lysate (Native)

  • Ca(2+)/calmodulin-dependent protein kinase phosphatase
  • CaMKPaseCaM-kinase phosphatase
  • EC 3.1.3.16
  • FEM-2
  • hFEM-2
  • KIAA0015POPX2CAMKP
  • Partner of PIX 2
  • PP2C phosphatase
  • Protein fem-2 homolog
  • protein phosphatase 1F (PP2C domain containing)
  • protein phosphatase 1F
  • protein phosphatase, Mg2+/Mn2+ dependent, 1F

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PPM1F