Human Protein phosphatase 1F ELISA Kit (Colorimetric)

ELISA: Human Protein phosphatase 1F ELISA Kit (Colorimetric) [NBP2-60587] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 1.56 - 400 ng/mL (example only; lot dependent)
• Sensitivity: 0.3 ng/mL (example only; lot dependent)

Human Protein phosphatase 1F ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1.56 - 400 ng/mL (example only; lot dependent)
• Sensitivity: 0.3 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 11.6% (example only; lot dependent)
• Intra-Assay: %CV < 6.7% (example only; lot dependent)
• Spike Recovery: 94 - 115% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PPM1F

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Protein phosphatase 1F ELISA Kit (Colorimetric)

• Ca(2+)/calmodulin-dependent protein kinase phosphatase
• CaMKPaseCaM-kinase phosphatase
• EC 3.1.3.16
• FEM-2
• hFEM-2
• KIAA0015POPX2CAMKP
• Partner of PIX 2
• PP2C phosphatase
• Protein fem-2 homolog
• protein phosphatase 1F (PP2C domain containing)
• protein phosphatase 1F
• protein phosphatase, Mg2+/Mn2+ dependent, 1F

Background

Protein phosphatase 1F is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Protein phosphatase 1F ELISA Kit (NBP2-60587). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PPM1F