PRMT7 Antibody

Western Blot: PRMT7 Antibody [NBP2-26135] - (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRMT7 Antibody Summary

• Description: Novus Biologicals Goat PRMT7 Antibody (NBP2-26135) is a polyclonal antibody validated for use in WB and ELISA. Anti-PRMT7 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PRFGEINDQDRTDR corresponding to internal region according to NP_001171753.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_061896.1; NP_001171753.1).
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRMT7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in lysates of HeLa and of mouse spleen (calculated MW of 78.5 kDa band according to human NP_061896.1 and 78.3 kDa band according to mouse NP_663379.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRMT7 Antibody

• [Myelin basic protein]-arginine N-methyltransferase PRMT7
• EC 2.1.1
• EC 2.1.1.125
• EC 2.1.1.126
• EC 2.1.1.321
• FLJ10640
• Histone-arginine N-methyltransferase PRMT7
• KIAA1933
• KIAA1933EC 2.1.1.-
• myelin basic protein-arginine N-methyltransferase
• PRMT7
• protein arginine methyltransferase 7
• protein arginine N-methyltransferase 7

Background

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT7 Antibody (NBP2-26135)(2)

Publications using NBP2-26135

• Gunes Gunsel G, Conlon TM, Jeridi A et al. The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD Nature communications 2022-03-14 [PMID: 35288557] (WB, Mouse)
• Lee JH, Cook JR, Yang ZH et al. PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine. J Biol Chem 2005-02-04 [PMID: 15494416]

Bioinformatics

• Gene Symbol: PRMT7
• Entrez:
  • 54496(Human)
  • 214572(Mouse)
  • 361402(Rat)