PPP2R4 Antibody

Western Blot: PPP2R4 Antibody [NBP1-51951] - Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R4 Antibody Summary

• Description: Novus Biologicals Goat PPP2R4 Antibody (NBP1-51951) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PPP2R4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GSLLPIHPVTSG corresponding to C-Terminus according to NP_066954.2, NP_821070.1.
• Epitope: C-GSLLPIHPVTSG
• Specificity: This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human, mouse and rat liver lysates and in HepG2 lysates (calculated MW of 36.8 kDa band according to NP_066954.2).Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Testis.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R4 Antibody

• EC 5.2.1.8
• MGC2184
• Phosphotyrosyl phosphatase activator
• PP2A phosphatase activator
• PP2A subunit B' isoform PR53
• PP2A
• PP2A, subunit B', PR53 isoform
• PPP2R4
• protein phosphatase 2A activator, regulatory subunit 4
• PTPA
• regulatory subunit B' (PR 53)
• serine/threonine-protein phosphatase 2A activator
• Serine/threonine-protein phosphatase 2A regulatory subunit 4
• Serine/threonine-protein phosphatase 2A regulatory subunit B'

Background

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R4 Antibody (NBP1-51951)(1)

Publication using NBP1-51951

• Gushiken FC, Patel V, Liu Y et al. Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling. J Biol Chem 2008-05-09 [PMID: 18334487]

Bioinformatics

• Gene Symbol: PTPA
• Entrez:
  • 5524 (Human)
  • 110854(Mouse)
  • 362102(Rat)
• Uniprot:
  • NP_821068.1(Human)