Recombinant Human PPIL2 Protein

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SDS-Page: Recombinant Human PPIL2 Protein [NBP1-41225] - PPIL2, 61.6 kDa (547aa) with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
0.5 mg/ml

Order Details

Recombinant Human PPIL2 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-527 of Human PPIL2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH

Details of Functionality
Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin..
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PPIL2
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
61.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human PPIL2 Protein

  • CYC4
  • cyclophilin, 60kDa
  • cyclophilin-60
  • Cyclophilin-like protein Cyp-60
  • CYP60
  • Cyp-60
  • EC 5.2.1.8
  • FLJ39930
  • hCyP-60
  • MGC33174
  • MGC787
  • peptidylprolyl cis-trans isomerase
  • peptidyl-prolyl cis-trans isomerase-like 2
  • peptidylprolyl isomerase (cyclophilin)-like 2
  • PPIase
  • Rotamase PPIL2

Background

PPIL2, which belongs to the cyclophilin family of peptidylprolyl isomerases, is an enzyme that plays an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Recombinant PPIL2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PPIL2