PPIL1 Overexpression Lysate (Native)

Images

 
Western Blot: PPIL1 Overexpression Lysate (Adult Normal) [NBL1-14651] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPIL1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPIL1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200015 and based on accession number NM_016059. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens peptidylprolyl isomerase (cyclophilin)-like 1 (PPIL1), mRNA.
Gene
PPIL1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PPIL1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPIL1 Overexpression Lysate (Native)

  • CYPL1cyclophilin-related gene 1
  • EC 5.2.1.8
  • hCyPX
  • MGC678
  • peptidyl-prolyl cis-trans isomerase
  • peptidyl-prolyl cis-trans isomerase-like 1
  • peptidylprolyl isomerase (cyclophilin)-like 1
  • PPIase
  • Rotamase PPIL1

Background

This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
MAB3777
Species: Hu, Mu, Rt
Applications: WB
MAB7670
Species: Hu
Applications: WB, ICC
H00002288-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P, IP
MAB2294
Species: Hu, Mu
Applications: WB, IHC, IP
NB120-3562
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF4094
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-15079
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19117
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-38604
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83890
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-14016
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-83889
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85358
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-87929
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3580
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-38905
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-14651
Species: Hu
Applications: WB

Publications for PPIL1 Lysate (NBL1-14651) (0)

There are no publications for PPIL1 Lysate (NBL1-14651).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PPIL1 Lysate (NBL1-14651) (0)

There are no reviews for PPIL1 Lysate (NBL1-14651). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPIL1 Lysate (NBL1-14651) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PPIL1 Products

Bioinformatics Tool for PPIL1 Lysate (NBL1-14651)

Discover related pathways, diseases and genes to PPIL1 Lysate (NBL1-14651). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PPIL1 Lysate (NBL1-14651)

Discover more about diseases related to PPIL1 Lysate (NBL1-14651).
 

Pathways for PPIL1 Lysate (NBL1-14651)

View related products by pathway.

Blogs on PPIL1

There are no specific blogs for PPIL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PPIL1 Antibody
NBP2-57062

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PPIL1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PPIL1