PP2C beta/PPM1B Antibody

Western Blot: PP2C beta/PPM1B Antibody [H00005495-D01P] - Analysis of PPM1B expression in transfected 293T cell line by PPM1B polyclonal antibody.Lane 1: PPM1B transfected lysate(52.60 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: PP2C beta/PPM1B Antibody [H00005495-D01P] - Analysis of protein-protein interactions between PPM1B and CHUK. HeLa cells were stained with anti-PPM1B rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

PP2C beta/PPM1B Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PPM1B (NP_002697.1, 1 a.a. - 479 a.a.) full-length human protein. MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAAGKSGSALELSVENVKNGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPKHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEYVKSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI
• Specificity: PPM1B - protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PP2C beta/PPM1B Antibody

• EC 3.1.3.16
• MGC21657
• PP2C beta
• Pp2c2
• PP2CB
• PP2C-beta
• PP2C-beta-X
• PP2CBmagnesium-dependent, beta isoform
• PPM1B
• protein phosphatase 1B
• Protein phosphatase 2C isoform beta
• protein phosphatase 2C-like protein
• protein phosphatase, Mg2+/Mn2+ dependent, 1B

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1B
• Entrez:
  • 5495(Human)
• Uniprot:
  • NP_002697.1(Human)