Potassium Channel Kv3.1 Antibody

Western Blot: Potassium Channel Kv3.1 Antibody [NBP1-42819] - Membrane preparations from HEK-293 cells untransfected (lane 1), transfected with Kv3.1 (lanes 2 and 4) and transfected with Kv1.2 (lane 3) were subjected to SDS-PAGE and were Western blotted with Kv3.1 Antibody (NBP1-42819). Lane 4 was probed with NBP1-42819 that had been preincubated with the peptide used to generate NBP1-42819.

Product Discontinued

Potassium Channel Kv3.1 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of rat Kv3.1 (within residues 350-400). [Swiss-Prot# P25122]
• Localization: Membrane; Multi-pass membrane protein.
• Specificity: Does not cross-react with Kv1.2 or Kv1.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Kv3.1 antibody is useful for Blocking/Neutralizing, Western blot and Immunoprecipitation. In WB a band can be seen at ~110 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, rabbit, bovine, canine and other vertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Potassium Channel Kv3.1 Antibody

• FLJ41162
• FLJ42249
• FLJ43491
• KCNC1
• KV3.1
• KV4
• MGC129855
• NGK2
• Potassium Channel Kv3.1
• potassium voltage-gated channel subfamily C member 1
• potassium voltage-gated channel, Shaw-related subfamily, member 1
• voltage-gated potassium channel protein KV3.1
• Voltage-gated potassium channel subunit Kv3.1
• Voltage-gated potassium channel subunit Kv4

Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995; Pongs, 1999; Blaine and Ribera, 1998; Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999). Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Potassium Channel Kv3.1 Antibody (NBP1-42819)(2)

Publications using NBP1-42819

• Chen C, Wang L, Rong X et al. Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats. Acta Pharm Sin B 2015-01-01 [PMID: 26579425] (WB, Rat)
• Zhou BY, Ma W, Huang XY. Specific antibodies to the external vestibule of voltage-gated potassium channels block current. J Gen Physiol;111(4):555-63. 1998-04-01 [PMID: 9524138] (WB, Human)

Bioinformatics

• Gene Symbol: KCNC1
• Entrez:
  • 3746(Human)
  • 16502(Mouse)
  • 25327(Rat)
• Uniprot:
  • P25122(Rat)