POFUT2 Antibody (OTI3A12) - Azide and BSA Free

Western Blot: POFUT2 Antibody (OTI3A12) - Azide and BSA Free [NBP2-73512] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POFUT2.

Flow Cytometry: POFUT2 Antibody (OTI3A12) - Azide and BSA Free [NBP2-73512] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by POFUT2 antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

POFUT2 Antibody (OTI3A12) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human POFUT2(NP_598368) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POFUT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:1000
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for POFUT2 Antibody (OTI3A12) - Azide and BSA Free

• C21orf80
• chromosome 21 open reading frame 80
• FUT13EC 2.4.1.221
• GDP-fucose protein O-fucosyltransferase 2
• KIAA0958
• O-FucT-2
• Peptide-O-fucosyltransferase 2
• protein O-fucosyltransferase 2

Background

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POFUT2